Why does my rabbit serum "gel"?
I.McFarlane at icrf.icnet.uk
Thu Apr 9 07:03:46 EST 1998
I'm not an expert but I would guess that there is still some fibrinogen
left in the plasma which forms a fibrin clot. You can extract the serum
from the clot just pick it up and squeeze it!
In article <6gfddu$knr at metro.usyd.edu.au>, Phil Robinson
> When we bleed a rabbit we sit the blood in a falcon tube for 1-4 hrs,
> then put it in the cold room overnight. In the morning we gently remove
> the clotted cells. However, when we spin the serum to clean up the
> debris the serum occasionally "gels", meaning we lose two thirds of our
> precious serum as it is now like a ball of soft acrylamide. People I
> ask have never experienced this. However I have seen it many times over
> 10 years. We tried several different types of plastic and glass tubes.
> Currently we have one rabbit (our best one of course) where it occurs on
> every bleed.
> Any ideas on what is going on? How to prevent this? How to dissolve the
> serum clot?
> Help or advice would be appreciated.
> Phil Robinson, Sydney
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