DNA Ligase in 2nd Strand Buffer. Why?

Jeremy A. Guggenheim Guggenheim at cardiff.ac.uk
Wed Apr 15 11:21:08 EST 1998


Several manufacturers (e.g. Gibco BRL) use E. Coli DNA Ligase in their
second strand cDNA reaction buffers (along with the usual DNA
ploymerase and RNase H). They then say that the products formed will
be blunt-ended, double stranded cDNAs.

Does anyone know what the DNA ligase is there for?
And couldn't it cause concatemerization of the products?

********** The light at the end of the tunnel **********
******* is the headlight of an oncoming train! *******

Dr. Jeremy A. Guggenheim
Dept. of Optometry & Vision Sciences
Cardiff University
P.O. Box 905, Cardiff, CF1 3XF, Wales, UK
e-mail. guggenheim at cf.ac.uk
fax.    44-1222-874859




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