Dissolving Ciprofloxacin

Kiley R. Prilliman Kiley-Prilliman at ouhsc.edu
Wed Apr 15 21:42:47 EST 1998


> 1) How is Ciprofloxacin dissolved for use in tissue culture and
>         2) What is the concentration generally used in tissue culture
>
> I have Ciprox. powder (as oral capsules, 250 mg) but it dosen't dissolve
> in PBS, Water, 0.85% NaCl, RPMI 1640 (at 1 mg/ml). Injectible solution
> (i/v) is available as 2 mg/ml solution in 0.9% NaCl. Does oral capsule in
> powder form contain any additives (not mentioned on the packet)?
>
> Thanks
>
> CJ
>


Hello,

When I have used ciprofloxacin in the past, I dissolve it directly into
my RPMI (the concentration I have used with success for treatment of
mycoplasma is 19µg/mL).

Kiley






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