polyacrylamide gel with formamide added???

Rachael Woram rworam at uoguelph.ca
Wed Apr 15 09:13:36 EST 1998


I've recently been reading a protocol which involved making a 6%
polyacrylamide gel with 5.6M urea and 30% formamide in TBE. Has anyone
tried this? It is supposed to eliminate poorly defined bands and smearing
that happens with some microsatellites.





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