secondary bands after in vitro transcription
jschmitz at mail.mpiz-koeln.mpg.de
Wed Apr 15 05:29:22 EST 1998
I have a problem with my latest in vitro transcription assays.
After in vitro transcription of some DNA templates using T3 or T7
I detect additional bands on a denaturing agarose gel. These bands are
than the expected product and thus should be due to prematurely
Is there any protocol available to avoid premature termination during in
Is it possible to add compounds like DMSO into the in vitro
in order to melt RNA secondary structure and thus avoid premature
I will greatly appreciate any suggestion to overcome this problem.
(JuerGen is best expressed at 10 p.m in 10x beer)
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