screening clones by RT-PCR

Mike Smith mfs3k at virginia.edu
Thu Apr 16 13:29:01 EST 1998


I'm making stable transfectants that I need to screen for expression of the
transected gene.  The best way for me to screen is by RT-PCR.  Does anybody
has a quick and easy method to do this from a small number of cells in a
96-well plate?  I'd like to be able to do a large number of clones without
having to grow them up and prep total RNA from each clone individually.

Thanks,
Mike Smith
University of Virginia





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