DNA precipitation: EtOH vs. Isoprop

brett brett at BORCIM.WUSTL.EDU
Sat Apr 18 18:24:41 EST 1998


>Hi all,
>
>I have a question concerning the precipitation of DNA.  In some 
>situation one uses EtOH to do so and others call for Isoprop. What
>is the difference? I know that with Isoprop the pellet is much less
>salty than it is with EtOH. But what else? Why not Isoprop then all the
>time?
>
>Thanks in advance,
>Ricky 
>

Gee Ricky, I thought IPA left your DNA saltier, but that IPA used less volume
(and so is the method of choice for large samples). Yes, here it is, page 46
of Methods in Enzymology  V152.

Brett at borcim.wustl.edu




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