Very sensitive sulfhydryl assay?

[Roger Murphy]

Do you have access to a mass spec, or to a friendly mass spectroscopist?  You 
could derviatize with iodoacetamide or vinylpyridine and measure the mass 
after derivatization - gives you an exact measure of free sulphydryls, and it 
should be sensitive in the fmol-pmol range.  Of course, you'll need to ensure 
you can get a molecular ion for your protein before you derivatize.....

Cheers,

Roger

In article <cbystrom-1304982013360001 at cro-dialin-1.uoregon.edu>, 
cbystrom at darkwing.uoregon.edu (cory) wrote:
>Hi,
>
>I was wondering if anyone can point me in the direction of a very
>sensitive assay for the quantitation of free sulfhydryls.  Reaction with
>DTNB is just too protein intensive for the samples that I have available.
>
>A capillary electrophoresis assay would be especially nice but anthing will do!
>
>Thanks much,
>
>Cory
>cbystrom at darkwing.uoregon.edu



Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg,  Vic. 3084
Australia.

Tel  61-3-94965463
Fax  61-3-94965436
Email murphy_r at licre.ludwig.edu.au