Very sensitive sulfhydryl assay?
murphy_r at licre.ludwig.edu.au
Sun Apr 19 22:32:15 EST 1998
Do you have access to a mass spec, or to a friendly mass spectroscopist? You
could derviatize with iodoacetamide or vinylpyridine and measure the mass
after derivatization - gives you an exact measure of free sulphydryls, and it
should be sensitive in the fmol-pmol range. Of course, you'll need to ensure
you can get a molecular ion for your protein before you derivatize.....
In article <cbystrom-1304982013360001 at cro-dialin-1.uoregon.edu>,
cbystrom at darkwing.uoregon.edu (cory) wrote:
>I was wondering if anyone can point me in the direction of a very
>sensitive assay for the quantitation of free sulfhydryls. Reaction with
>DTNB is just too protein intensive for the samples that I have available.
>A capillary electrophoresis assay would be especially nice but anthing will do!
>cbystrom at darkwing.uoregon.edu
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Heidelberg, Vic. 3084
Email murphy_r at licre.ludwig.edu.au
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