Immunprecip. with anti-HIS6 ab

Thomas Urbig urbig at biokemi.su.se
Mon Apr 20 08:53:11 EST 1998


Hi,

I would like to do an immunprecip. of recombinant, HIS-tagged proteins.
Has anybody done this successfully with an ab directed against the
HIS6-part (especially with the Qiagen anti-HIS4/anti-HIS5 ab´s)? 
Could anybody give me a reference to a publication or a protocol to do so
to have an idea about the starting conditions.

Thanks in advance

Thomas



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