Q: site-directed mutagenesis of large DNA (>50 kb)

Tom Litjens tlitjens at gina.adelaide.edu.au
Mon Apr 20 01:43:45 EST 1998


Hello all;

I am interested in using site-directed mutagenesis to insert a 25-30 bp
sequence into  BAC clones which have insert sizes ranging from 75-200 kb. 
Any suggestions concerning methods and/or kits would be most welcome. 
Thanks in anticipation,

Tom Litjens



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