TA cloning vector

Stephen Billington billing at vetsci.microvet.arizona.edu
Mon Apr 20 14:34:07 EST 1998


Does anyone know of a company that still sells TA cloning vector (with
an A overhang) without the kit (cells, ligase, dNTPS, kitchen sink,
etc.)?  All the companies that used to sell them (Clontech, Invitrogen,
etc.) have gone to the kit format and at >$250/20 rxns, the cost is
prohibitive.

We have tried making TA vector as per the Biotechniques protocol of a
couple of years ago, but have not had much success.

TIA

SJB




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