DNA precipitation: EtOH vs. Isoprop

Gail Sudlow g-sudlow at uiuc.edu
Mon Apr 20 17:47:53 EST 1998


In article <353943EC.103F at nospam.ipk-gatersleben.de>,
boernke at nospam.ipk-gatersleben.de wrote:

> Hi all,
> 
> I have a question concerning the precipitation of DNA.  In some 
> situation one uses EtOH to do so and others call for Isoprop. What
> is the difference? I know that with Isoprop the pellet is much less
> salty than it is with EtOH. But what else? Why not Isoprop then all the
> time?

I think I have heard that the pellet from isoprop is more glassy looking
and harder to see.  Gail>

 
> Thanks in advance,
> Ricky 
> 
> ******************************************************************
> 
> Frederik Boernke
> Research Group of  Molecular Plant Physiologie
> Institute for Plant Genetics and Crop Plant Research (IPK)
> Corrensstr. 3
> 06466 Gatersleben
> Tel.  039482 -5 321
> Fax. 039482 -5 515
> e-mail: boernke at ipk-gatersleben.de
> http://www.ipk-gatersleben.de



More information about the Methods mailing list