anti-His antibody

[Lars Komorowski]

Flore Bellefontaine schrieb in Nachricht ...
>Hi!
>Did someone ever detect with an anti-his antibody a recombinant protein
>purified from pet15B (Novagen)?  The recombinant prot presents a six
>histidines tag at the NH3-term.
>I have been using unsuccesfully the penta-His antibody from Qiagen in
>ELISA, Dot Blot under native and denaturing conditions and in Western Blot
>aswell. Do you have any experience using that antibody?
>Any kind of help is wellcome, thank you in advance,
>
>Flore Bellefontaine


I have used the Tetra-His-Ab from Qiagen. It works but is a little bit too
sensitive.