HELP: METHOD FOR ATP PURIFICATION NEEDED

Zophonias O. Jonsson zjons at vetbio.unizh.ch
Wed Apr 22 10:51:32 EST 1998


In article <Pine.OSF.3.96.980422174615.22487A-100000 at sanger.otago.ac.nz>,
David Ackerley <david at sanger.otago.ac.nz> wrote:

> Hi there
> 
> I am presently attempting ATP/PPi exchange assays, a crucial step of which
> is the collection of ATP by binding to activated charcoal. Unfortunately,
> the degree of binding appears to vary anywhere from 5-25%, not greatly
> aiding the sensitivity of the assay... Does anyone out there know of a
> better means for collection of ATP, that would still allow unincorporated
> PPi to be washed off? It is pretty crucial that either a consistent or
> else a very high percentage of the Atp present be retained.
> 
> 
> Thanks in Advance,
> 
> Dave

You can easily separate ATP and PPi by thin layer chromatography.  It
should then be possible to cut out the ATP / PPi containing spots and
solubilize them, or whatever...  It's just an Idea but anyway...

Zophonias

_____________________________________________________________________
Zophonias O. Jonsson
Institut fur Veterinarbiochemie               Tel: (41-1)-635-54-75
Universitat Zurich-Irchel                     Fax: (41-1)-635-68-16
Winterthurerstrasse 190
CH-8057 Zurich
Switzerland
_____________________________________________________________________



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