the amount of 35S-UTP to synthisize riboprobe

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Wed Apr 22 19:12:52 EST 1998


Does any one have experience to synthesize 35S-riboprobe with 35S-UTP with
RNA transcriprion kit (Starategene, 200340)? Otherwise, in 25ul reaction
containg 100ng DNA, how much uCI of label should be used?

Jun-ichi Aikawa, Ph.D.
University of California, San Diego





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