Labeling PCR primers

Hiranya Roychowdhury hroychow at NMSU.EDU
Wed Apr 22 14:52:16 EST 1998


At 11:23 AM 4/22/98 +0000, John Fellers wrote:
>I am looking for a protocol to label PCR primers with P32.  I want to
>use the primers to identify amplified products.  Thanks in advance.
>
>

Exchange reaction with T4-PNK, using [gamma-32P]ATP. This will label the
ends. Sambrook et al. or any other manual should help.


Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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