RNA transfections

Paul Gorry gorry at burnet.edu.au
Wed Apr 22 02:19:49 EST 1998


Dear molecular/cellular biologists,
I'm posting this message on behalf of a collegue of mine who is trying to
transfect HeLa and Jurkat cells with circularized RNA.  She is using the
Gibco-BRL DMRIE-C protocol which only applies to adherent cells, and
hasn't had much luck yet.  We would be very interested to hear from anyone
who has optimized this RNA transfection protocol, or used any other
protocols for non-adherent cells.

Thanks in advance,

Paul Gorry.
gorry at burnet.edu.au
Macfarlane Burnet Centre for Medical Research



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