HELP: Coli is rearranging recombinant plasmid

Alexey Merz alexey at webcom.com
Wed Apr 22 19:10:00 EST 1998


clemen suter-crazzolara wrote:
> On Wed, 22 Apr 1998 21:12:01 +0100, Alexey Merz  said:

> >Great vectors are the pWKS/pWSK vectors. These vectors are _identical_
> >to the pBluescript plasmids (same polylinker, F1 ori, etc) but have a
> >low copy origin and -better yet- WSK129 and WKS130 are Kanamycin
> >resistant instead of Amp resistant, meaning zero satellite colonies.

> wasn't there a new antibiotic which functions like ampicillin
> (ie is a target for beta-lactamase), which is much more stable
> and also results in much fewer satillites ?

Yeah, carbenicillin. Make 100 or 200 mg/ml stock in 50% EtOH, store -20,
use at similar concentration as Amp. One problem: expensive.

Another option: ampicillin/methicillin mixture. Anybody try this?
With what results? 
..........................................................................
Alexey Merz | URL: http://www.webcom.com/alexey | email: alexey at webcom.com
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            | "I had also thought that Vanna was the ultimate TV creature,
            | a Dada artist who does not speak but methodically destroys
            | language by revealing cliches letter by letter, day after
            | day, year after year."                      -Andrei Codrescu



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