basic question on: transcription factor-DNA seq. recognition

Guido Hooiveld g.j.e.j.hooiveld at farm.rug.nl
Fri Apr 24 00:55:16 EST 1998


I was just wondering about the following:

A transcription factor, e.g. SREBP (sterol regulatory element-binding protein) 
enhances transcription of genes by recognizing a SRE (sterol regulatory 
element), a 10 bp seq, in its promotor.

Schematically, one can draw the following (SRE consensus sequence in small 
capitals):

5'-NNN atc acc cca cNN NNN-3' (sense DNA strand, strand corresponds with mRNA)
3'-NNN TAG TGG GGT GNN NNN-5' (complementary DNA strand)



Is it, therefore, also possible that a inverted SRE sequence enhances 
transcription of a gene in the presence of SREBP, e.g. in this situation?:

5'-NNN GTG GGG TGA TNN NNN-3' (sense DNA strand)
3'-NNN cac ccc act aNN NNN-5' (complementary DNA strand, containing 5'-3' SRE 
seq.)

It would be helpful to know your ideas regarding this theoretical question.


Please post here, or send an e-mail directly:
g.j.e.j.hooiveld at farm.rug.nl


Thanx in advance!

Guido Hooiveld
University of Groningen
Dept. Internal Medicine
The Netherlands




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