the amount of 35S-UTP to synthisize riboprobe

Ed Frank operon at wt.net
Thu Apr 23 20:48:27 EST 1998


We typically use 10ul of 35S-UTP (10mCi/ml, Amersham) in a total
reaction volume of 30 ul with good result.  The amount of DNA template
in our reactions is closer to 500 or 600ng than to 100ng.  Best of
luck.

Ed Frank
Baylor College of Medicine



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