Unusual behaviour of protein on SDS-PAGE gels - any suggestions?

Ian A. York iayork at panix.com
Fri Apr 24 09:38:41 EST 1998


In article <das1002-2404981254250001 at ngay1.bioc.cam.ac.uk>,
David <das1002 at cam.ac.uk> wrote:
>
>if not boiled before loading, it runs as one band, whereas if one boils it
>first, it runs as two bands: one of these runs the same as the band one

This sounds a little like the behaviour of MHC class II; it reflects the
different conformations of the dimer with and without peptide added
(peptide stablilizes and slightly changes the conformation).  It's not
identical behaviour, but perhaps it's a starting point.

Ian

-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England



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