DNA Polymerase

Vladimir Svetlov svetlov at oncology.wisc.edu
Fri Apr 24 13:07:29 EST 1998

In article <6ho0vn$oti$1 at vixen.cso.uiuc.edu>, "Troy Jesse"
<tjesse at students.uiuc.edu> wrote:

> I am posting to find out from folks on this NG what types of polymerases
> people have had good results with when running PCR.
Good results depend on what you are after. Good overall polymerase for PCR
cloning is Pwo, for SD mutagenesis - Pfu, for random mutagenesis and 3'
mismatch testing - Taq. Some applications like >10 kb amplifications
require mixes. If you can give some specifics of the intended PCR we might
be able to narrow down the spectrum.


Vladimir Svetlov, Ph. D.
McArdle Lab for Cancer Research
Dept. Oncology
1400 University Ave.
Madison, WI 53706

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