HELP: METHOD FOR ATP PURIFICATION NEEDED

Vladimir Svetlov svetlov at oncology.wisc.edu
Fri Apr 24 13:02:35 EST 1998


In article <Pine.OSF.3.96.980422174615.22487A-100000 at sanger.otago.ac.nz>,
David Ackerley <david at sanger.otago.ac.nz> wrote:

 
 > I am presently attempting ATP/PPi exchange assays, a crucial step of which
 > is the collection of ATP by binding to activated charcoal. Unfortunately,
 > the degree of binding appears to vary anywhere from 5-25%, not greatly
 > aiding the sensitivity of the assay... Does anyone out there know of a
 > better means for collection of ATP, that would still allow unincorporated
 > PPi to be washed off? It is pretty crucial that either a consistent or
 > else a very high percentage of the Atp present be retained.

ATP can be qualitatively precipitated by ethanol in presence of Ca++. It's
been a while since I've done it (about 7 years) but such protocol does
exist. Let me see if I can figure out where in hell I got it in the first
place. Not much help, heh?

Regards,
V.

-- 
Vladimir Svetlov, Ph. D.
McArdle Lab for Cancer Research
Dept. Oncology
UW-Madison
1400 University Ave.
Madison, WI 53706



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