anti-His antibody

[Dima Klenchin]

In article <6hhsvh$o6m$1 at gwsun.medinf.mu-luebeck.de>, "Lars Komorowski" <larskomo at physik.mu-luebeck.de> wrote:
>
>Flore Bellefontaine schrieb in Nachricht ...
>>Hi!
>>Did someone ever detect with an anti-his antibody a recombinant protein
>>purified from pet15B (Novagen)?  The recombinant prot presents a six
>>histidines tag at the NH3-term.
>>I have been using unsuccesfully the penta-His antibody from Qiagen in
>>ELISA, Dot Blot under native and denaturing conditions and in Western Blot
>>aswell. Do you have any experience using that antibody?
>>Any kind of help is wellcome, thank you in advance,
>>
>>Flore Bellefontaine
>
>
>I have used the Tetra-His-Ab from Qiagen. It works but is a little bit too
>sensitive.
>

There is no such thing as "too sensitive' with whatever antibody. Dilution
sets a dynamic range of detection. 

Y.