Zeocin selection in tissue culture cells

Simon Dawson Simon.Dawson at nott.ac.uk
Mon Apr 27 05:35:49 EST 1998


Hi,
    We are having a problem selecting tissue culture cells using the Zeocin
antibiotic from Invitrogen. We think it's a problem with the salt in the
DMEM we are using (6.4g/l - Invitrogen recommend no more than 5g/l).
    Does anyone out there have much experience with Zeocin? What type of
media (and from what supplier) are people using with Zeocin? Just out of
interest, does anyone know the mechanism of Zeocin inactivation by NaCl?
    Thanks for any help that people can give.


            Simon.

--
Dr. Simon Dawson
Laboratory for Intracellular Proteolysis
Molecular and Cellular Biology Section
School of Biomedical Sciences
The Medical School
Queen's Medical Centre
Clifton Boulevard
Nottingham
NG7 2UH
U.K.

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TEL:+44 (0)115 9249924 ex. 44787
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Email:Simon.Dawson at nott.ac.uk
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