granulosa cell RNA

[r.l.bates]

I'm trying to extract RNA from human granulosa cells collected 
during oocyte aspiration for IVF. At the moment I am using the 
Chomaski and Sacchi method after extracting the granulosa cells. 
 
The first time I tried extracting RNA I cultured the cells first 
which gave RNA, now I have just been collecting the cells and 
going straight to RNA extraction and getting nowhere.
 
Do I need to culture them first or do I need to get hold of the 
oocyte aspirates more quickly and put them on ice?

Any suggestions would be appreciated.

Thanks
R.L.Bates

Depts of Obstetrics and Gynaecology, and Molecular and Cell 
Biology,
University of Aberdeen, Scotland, UK.