Staining fungal nucleoli
A.R.Ganley at massey.ac.nz
Tue Apr 28 23:20:43 EST 1998
I am attempting simple 'ole light microscopy, looking for nucleoli in my
fungi, but so far haven't met with the greatest success.
I have been using smears of fungal protoplasts that I fix in either
methanol or Carnoy's fluid (1 acetic acid:3 ethanol), and then stain
with 0.2% methylene blue mixed with saturated sodium borate.
Occasionally I get a few nuclei that stain up with nucleoli (or what I
presume are nucleoli), but its pretty inconsistent, and only a very few
cells stain properly.
Does anyone have any experience with this sort of work (especially with
fungi), or any alternative methods (I don't want to go into antibody
work or anything like that), as this isn't really my area.
E-mail to me.
More information about the Methods