quantitative pcr

[Jun Zhang]

Competitve RT-PCR is the choice.

good luck

mark
> 
> which methods would you recommand for quantitative RT-PCR? We have to quantitategene expression of a low expressed gene out of blood cells of children (few material) I'd like to quantitate without a housekeeping gene, eventually with densiometry.
> 
> could you help me
> thanks
> 
> --
> _________________________________________________
> 
> Bertram Feil <feilbert at sun2.ruf.uni-freiburg.de>
> Lorettostr. 62
> 79100 Freiburg
> 
> Tel. 0761/4002213
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>