Boiling ethidium bromide gel

Clemens Suter-Crazzolara un691cs at genius.embnet.dkfz-heidelberg
Tue Apr 28 03:18:19 EST 1998


On 25 Apr 1998 17:35:59 +0100, Wolfgang Schechinger  said:

>Hi Hiranya!

>You just boil your gel after taking the photo? - great! What 
>about an increasing background by DNA? is there any?

>Wolfgang 

>> I do it all the time and EtBr stays put. This way, I've been able to
>> reuse agarose at least 5 times without having to add significant
>> amts of EtBr. The savings are substantial too. This, of course, is
>> only for analytical purposes.

I would be careful about this method. after a while the resolution
becomes quite bad, because the agarose starts breaking down.
It is probably better to buy a cheaper agarose for quicky gels.

clemens




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