Kozak in protein expression

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Tue Apr 28 14:55:58 EST 1998


As I understand the function of the kozak consensus element, it 
enhances binding of the ribosome and thus translation. It shouldn't 
affect transcription to my knowledge.

Wo

> Can the Kozak site of a cDNA put into an expression plasmid eg
> pcDNA3.1 interfere with or be detrimental to transcription from the
> proper Kozak motif upstreams of the 5¦tag sequence?
> 
> 
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Wolfgang Schechinger         
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