Kozak in protein expression
wgschech at med.uni-tuebingen.de
Tue Apr 28 14:55:58 EST 1998
As I understand the function of the kozak consensus element, it
enhances binding of the ribosome and thus translation. It shouldn't
affect transcription to my knowledge.
> Can the Kozak site of a cDNA put into an expression plasmid eg
> pcDNA3.1 interfere with or be detrimental to transcription from the
> proper Kozak motif upstreams of the 5¦tag sequence?
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