cDNA synthesis in high G/C regions

Jim jhughes at immsvr.jr2.ox.ac.uk
Wed Apr 29 07:02:34 EST 1998


Hi everbody

Does anybody have a good protocol for reverse trancription over CpG
islands , the %GC in this region is extremely high . Something like a
high temperature reverse transcriptase would be convenient, if such a
thing existed.

many thanks
Jim
--
Jim Hughes
Institute of molecular medicine
John Radcliffe Hospital
Headington
Oxford
OX3 9DU





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