NaCl and culture medium

ruben.varela-calvino at kcl.ac.uk ruben.varela-calvino at kcl.ac.uk
Wed Apr 29 10:23:35 EST 1998


Hi everybody
I'm trying to express my recombinant protein using the LB medium with little
suscess by the moment. Now I'm trying 2xYT and SuperBroth and I realize that
the amount of NaCl people use here to prepare the LB medium (10 gr/lt) was
twice of 2xYT/SuperBroth (5 gr/lt), but cells seems to growth without any
problem
I have one question: can this affect the production of recombinant
proteins?
Ruben

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