Help with Mycobaterial DNA Prep
d.xu at genesis.co.nz
Thu Apr 30 22:40:07 EST 1998
I am currently attempting to prep plasmid DNA from transformed mycobateria, but
am experiencing annoying genomic DNA contamination in the final product.
Does anyone out there have, or have reference to, a protocol for purifying
nice, clean plasmid DNA from transformed mycobacteria?
My current protocol involves simple mycobacterial lysis via SDS and Proteinase
K, the simple CTAB and phenol/chloroform extraction of DNA.
Anyway help would be greatly appreciated.
<d.white at genesis.co.nz>
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