salmon sperm DNA for ppt'n

ELADER at AMBION.COM ELADER at AMBION.COM
Thu Apr 30 18:23:52 EST 1998



> Can I use salmon sperm DNA as a carrier to assist in EtOH precipitation
> of 32-P labelled DNA? I usually use glycogen, but we ran out, and I
> don't think we have tRNA to use.

If all you're doing is using the DNA as a probe it's OK. If the salmon sperm
DNA is not sheared, it may be difficult to get into solution if you dry the
pettel to much. use 1-10 ug per ml.

Eric

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