how to kill BamH1

[Mark Silby]

Hi there

What temperature are you using to "kill" the enzyme?  With the heat stable
enzymes, I have used 85 degrees celcius (10 minutes) with some success.  But
the downside is I am always a little concerned for my precious DNA in these
situations!  I prefer to use a gel extraction kit these days, as they are
quite quick and very reliable.

mark.

AKi Hoji wrote in message ...
>Dear,
>
>Are there any easy ways to eliminate BamH1 in a digestion soup ?  This
>tough buy appears to be heat-stable, and one has to perfrom gel
>purification or phenol-chroloform extraction of the digest. Any
>interesting methods will be appreciated !!
>Aki Hoji (akhst7+ at pitt.edu)
>University of Pittsburgh