In situ hybridization methods

Ira M. Herman, Ph.D. IHERMAN at OPAL.TUFTS.EDU
Thu Apr 30 12:32:58 EST 1998


We are looking for high-specificity, high-sensitivity protocols for
in situ hybridization of low abundance transcription factor for analysis
in frozen sections or paraffin.  Can anyone share with us their
experience. We are leaning to 33P-labeled probes but has 
anyone compared to the new kit from NEN (TSA Kit) using biotin-
tyramide??

RSVP

Ira M Herman, PhD
Professor 
Cell and Molecular Physiology
Tufts University School of Medicine
Boston MA 02111



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