Methods April 1998 Archives by date

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Starting: Wed Apr 1 00:17:44 EST 1998
Ending: Thu Apr 30 22:40:07 EST 1998
Messages: 733

• [Q] atomic biology   ÃÖ ¿õ
• Has anyone used fluorescein-5-maleimide to label a peptide?   Roger Murphy
• Has anyone used fluorescein-5-maleimide to label a peptide?   Roger Murphy
• definition of Gene Cloning   David Hagerberg
• Ligate, blunt ending and ligate   foc
• Separation of ss and ds DNA?   Christine Haworth
• Separation of ss and ds DNA?   Frank Maiwald
• SiO2???   Dr. Duncan Clark
• long-PCR   Andrew Warkman
• [Q] primer design   brett
• fire fly luciferase?   shangzd at public.bta.net.cn
• ABI-labelled primers develop extra peaks   Flemming Larsen
• How to prevent melting of short DNA duplexes   Kristian Hobolt Jensen
• Glycerol-3-Phosphate detection   CHRIS SMALL
• short fragment-PCR   Volker Seibert
• [Q] primer design   Curtis R. Altmann
• (no subject)   r.elble
• Ligate, blunt ending and ligate   John Richard Seavitt
• Q: remove enzyme with antiserum?   Dr E. Buxbaum
• Help locating Molecular Research Reagents Inc.   Rob Kirkpatrick
• Protein Renaturation   Richard Vernell
• Problem with TCA precipitation !correction!   Wolfgang Schechinger
• primer design   Tom Chappell
• IPTG   Silke Beismann
• Protein Renaturation   thierry.baussant at pierre-fabre.com
• long-PCR   Dr. Duncan Clark
• PCR Mistake   ddsyr at PUBLIC.EAST.CN.NET
• Blotting from Acrylamide Sequencing Gel   Dr Mick Jones
• Perseptive Biosystems' Streptavidin-coated beads   Deborah Wilkinson
• assay for free cysteines   Yu Wai Chen
• PCR Mistake   Chris Boyd
• GAL4 Ab for IF?   Andy Saurin
• polymerase I promoter   Nadia Naffakh
• BioMag Streptavidin magnetic particles: reposted   Deborah
• Q: hybridoma culture ?   Dimitri Harmegnies
• looking for an antibody library   Dimitri Harmegnies
• The Main Metabolic Pathways   Pieter
• IPTG   Andy Phillips
• RNA polymerase I promoter   Nadia Naffakh
• Q:PCR labeled with 32P?   January Weiner
• Blotting from Acrylamide Sequencing Gel   Wolfgang Schechinger
• PEG8000   Adelino Canario
• PCR Mistake   Koen De Smet
• assay for free cysteines   Wolfgang Schechinger
• question   Jose Alfredo Iglesias Jimenez
• Add A Link....Make Money!!!!!!!   evbechqxmakemoney at yuocanberich.net
• PEG8000   C.S. Wilding
• Blotting from Acrylamide Sequencing Gel   Warren Gallin
• Mutagenesis   Vladimir Svetlov
• Methods for quantification of gene expression   Zhencai Wu
• Help locating ZYNAXIS   Stacy Ferguson
• PCR Mistake   Bryan L. Ford
• X-ray crystal structure   Ivan Delgado
• primer design   Beth Wapelhorst
• filter sterilized vs. autoclaved water for PCR   Wolfgang Schechinger
• filter sterilized vs. autoclaved water for PCR   Dave Knaebel
• RT Assay washer   bbeitzel at biomail.ucsd.edu
• In situ with DIG-cRNA   Timur Yarovinsky
• assay for free cysteines   Petter
• HPLC problems   Siembieda family
• ORF finder   USER
• assay for free cysteines   Roger Murphy
• GC vs stability   Michael O'Donohue
• 6-methyl purine deoxyriboside   Didier.Wion at ujf-grenoble.fr
• In situ with DIG-cRNA   Frank Maiwald
• Methods for quantification of gene expression   Simon Mauch
• Methods for quantification of gene expression   Simon Mauch
• COS Transfections - DEAE or Lipofectin?   Chris Boyd
• Substrate for Alkaline Phosphatase   Klaus Lun
• EcoRI methylation   jheus at pharming.nl
• Biotinylation of plasmid DNA   Krzysztof Wasowicz
• protein/RNA isolation.   Jean-Christophe Roux
• Mutagenesis   Claudia Sutton
• HPLC problems   Peter
• filter sterilized vs. autoclaved water for PCR   Andrew Warkman
• Protease site prediction   MV. Hayes
• species homologue or new isoform   Soenke Behrends
• PCR Mistake   Elizabeth Rogers
• Flaws in this protocol? (TCA-extraction-yeast)   zaxxon
• Green Fluorescent Protein Purificaton Short Course   Daniel Gonzalez
• sequence analysis software   Robert Greenhall
• RNA Loading   Matthew Beyers
• protein/RNA isolation.   Jonathan E. Cohen
• Hexamer   Cindy boettger
• Nasty sequencing problem   Joseph C. Bagshaw
• Hexamer   Dom Spinella
• PCR Mistake   Bryan L. Ford
• Tubes/flasks for Beckman JLA10.500?   Wolfgang Schechinger
• protein/RNA isolation.   John R. Thompson
• PCR Mistake   John R. Thompson
• Flaws in this protocol? (TCA-extraction-yeast)   John R. Thompson
• F9 cells experience?   Marieke R. Koedood Zhao
• (none)   chenln at PUBLIC.BTA.NET.CN
• limits to restriction digestion?   Bob Steinberg
• species homologue or new isoform   Vladimir Svetlov
• Protease site prediction   Thomas L Selby
• protein/RNA isolation.   Peter
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"?   Dima Klenchin
• Biotinylation of plasmid DNA   Dima Klenchin
• Electroporation of sea urchin eggs   Dima Klenchin
• Drug tests?   Mexdoctor
• Ligate, blunt ending and ligate   Markus Piotrowski
• Q:PCR labeled with 32P?   Markus Piotrowski
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffe   Wolfgang Schechinger
• Antisense oligos (was: none)   Wolfgang Schechinger
• Chemical modification of Histidines   mark mcclain
• It is true   frfutlfp at hello.com
• northern blots   Cecilia Santos
• Immunoprecipitation of nuclear protein   AlPiEs
• looking for an antibody library   Elizabeth Chen
• question   Jose Alfredo Iglesias Jimenez
• limits to restriction digestion?   John Richard Seavitt
• Ambion, QuantumRNA kit   lattier at ibm.net
• X-ray crystal structure   greg at franklin.burnham-inst.org
• Ambion, QuantumRNA kit   ELADER at AMBION.COM
• species homologue or new isoform   s535290 at aix1.uottawa.ca
• Petroleum available   Julian Goldberg
• solubilizing nuclei   Wolfgang Schechinger
• Request for source of tissue plasminogen activator (tPA) ELISA kit   Ouyang Hongsheng
• ignore   first last
• Nasty sequencing problem   Alexander Kraev
• SiO2/Merlin miniprep   Chris Kafer
• Too high Tris-HCl in stacker SDS-PAGE a problem?   zaxxon
• Too high Tris-HCl in stacker SDS-PAGE a problem?   Ian A. York
• Electroporation of sea urchin eggs   Robert W. Zeller
• TA ligations - is Invitrogen the answer?   Andrew O'Neill
• a-AminoNitrogen   tsir
• filter sterilized vs. autoclaved water for PCR   Richard P. Grant
• 6-methyl purine deoxyriboside   Didier.Wion at ujf-grenoble.fr
• solubilizing nuclei   Martin Offterdinger
• Autoradiography/chemiluminesence - methods   carson at vision.net.au
• 6-methyl purine deoxyriboside   John Hegarty
• TA ligations - is Invitrogen the answer?   Heinz J. Schaefers
• Methanol in ethanol   Doug Johnson
• HYH   Yihua Han
• pEGFP   Yihua Han
• RNA Loading   Lubomir Sokolov
• Plasmid Deletions?   Ed Dunphy
• IPTG   Vladimir Svetlov
• pelleting canine pancreatic microsomes from in vitro translation rxn   perrinr1 at pilot.msu.edu
• pEGFP   Todd Serulneck
• E. coli strain KC8 request   Frederik Boernke
• IPTG   Joel Gagliardi
• Plasmid Deletions?   R. John Lye
• northern blots   ELADER at AMBION.COM
• Blotting from Acrylamide Sequencing Gel   ELADER at AMBION.COM
• Methods for quantification of gene expression   ELADER at AMBION.COM
• Methanol in ethanol   Richard Kerr
• Proteolytic map, freeware or web site?   Zeev Pancer
• Electroporation of sea urchin eggs   Dima Klenchin
• Heard of Biomedical Specialties?   gmunday at direct.ca
• Proteolytic map, freeware or web site?   Mathew Woodwark
• Long PCR of genomic DNA   John Fellers
• Thyroglobulin   Rob
• northern blots   January Weiner
• DNA electrophoresis nightmare   Joel Gagliardi
• Ameroid Constrictor   Shi-Dong Jia
• microarray hybridization protocol   Todd Martinsky
• taxotere/docetaxel source   Tobias Baskin
• TA ligations - is Invitrogen the answer?   soren k rasmussen
• Muristerone A   P. Gill
• RNA/Protein isolation   Jean-Christophe Roux
• DNA electrophoresis nightmare   Wolfgang Schechinger
• Updated NIH grant (PHS398) templates available for Mac Office 98   Dennis Templeton
• reusing plasmid maxiprep columns   Wolfgang Schechinger
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"?   Dennis Templeton
• Proteolytic map, freeware or web site?   Uncle S
• FISH   Mr. Z Mohamed
• help!   jpzhang at CUHK.EDU.HK
• need AMV, or M-MLV DNA   cll
• Thyroglobulin   Jakobs
• Proteolytic map, freeware or web site?   Uncle S
• GS antibodies   Yair M. Heimer
• probe specificity for northerns gel quantification   Dr.Chandrasekhar Gujuluva
• pEGFP   Yihua Han
• a-AminoNitrogen   tsir
• Activated Charcoal...What is it?   Phil Robinson
• Why does my rabbit serum "gel"?   Phil Robinson (delete_me)
• need AMV, or M-MLV DNA   Dr. Duncan Clark
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"?   theodorn at medlib.georgetown.edu
• reusing plasmid maxiprep columns   Dr. Duncan Clark
• thermo-cycler   Jørgen Borrebæk
• Your Search Engine Listing   list at LISTME.COM
• cross-reactivity HA epitope in adenovirus?   Danny Altschuler
• Southern info   John Korte
• Question about Strandase   AAAACc
• DNA electrophoresis nightmare   Dom Spinella
• probe specificity for northerns gel quantification   Dom Spinella
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"?   noone
• single stranded ladder   dlarkin
• pEGFP   Eugene
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"? <-- SOLVED   Dima Klenchin
• transient transfection????   Nevena Karaman-Jurukovska
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"?   Dr E. Buxbaum
• B12 mutant strain   Dr. Peter Gegenheimer
• X-gluc, any cheaper source   Chittibabu Guda
• B12 mutant strain   Ke-Xue Huang
• B12 mutant strain   Cornelius Krasel
• Filamentous E.coli   Rich L
• Southern info   Vladimir Svetlov
• DNA electrophoresis nightmare   Vivian Miao
• your web site...   planet at search-engine-help.com
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"? <-- SOLVED   noone
• Long PCR of genomic DNA   Bryan L. Ford
• Plasmid Deletions?   Claudia Sutton
• cell surface glycoproteins   Roberto Gualtieri
• Filamentous E.coli   Lesley Robertson
• Filamentous E.coli   Arnoud van Vliet
• dialysis product needed   Clemens Suter-Crazzolara
• protien/protein interaction   B.Faucon
• Why does my rabbit serum "gel"?   Ian McFarlane
• Lac Z staining   Dr.W.H. Colledge
• DNA polymerases for PCR   Joana Perdigao
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"?   Dennis Templeton
• Updated NIH grant (PHS398) templates available for Mac Office 98   Dennis Templeton
• Gene Editor System   Marcel Hoffmann
• reusing plasmid maxiprep columns   theodorn at medlib.georgetown.edu
• looking for antibody   Ling Ren
• enzymes removing methyl groups from DNA (EcoRI sites)   jheus at pharming.nl
• expression array   Bernd Giebel
• protien/protein interaction   Vladimir Svetlov
• Would like to know whether the idea will work!   Jaishree Muppala Chittoor
• enzyme activity in guanidine-HCl?   skorycd at ncaur.usda.gov
• DNA electrophoresis nightmare   D. KIM
• Hot Adult Site With Awesome Links!   adultwebsites at HOTMAIL.COM
• air compressor   zhanglin lin
• Activity assay for caspase 8   tohko iida
• DNA polymerases for PCR   Linda Western
• separating eosinophils   jpzhang at CUHK.EDU.HK
• dialysis product needed   Jorg Kirberg
• a-AminoNitrogen   Mark Schoenbeck
• Why does my rabbit serum "gel"?   Thomas R. Anderson
• Q: best western membrane?   Ted Michelini
• PCR using yoghurt sample   g9711278 at inhavision.inha.ac.kr
• taxotere/docetaxel source   3Dom
• enzyme activity in guanidine-HCl?   Vladimir Svetlov
• test   name
• Best place to get polyclonal Ab?   Dr. Robert Rumpf
• Best place to get polyclonal Ab?   Al Lammers
• looking for antisulfotyrosine!   Richard Converse
• Assembling EST sequences   Simon Hoffenberg
• Would like to know whether the idea will work!   Frederik Boernke
• Best place to get polyclonal Ab?   R. John Lye
• Casein problem for a solution   S. H.
• Oligos Central - directory of oligonucleotide synthesizing companies   WebMaster
• hsp and immunity   Menoret
• About thesis writing   Wilson
• Lyght Cycler Real time PCR request of information   Giuseppe Palumbo
• Lyght Cycler request of information 2   Giuseppe Palumbo
• Dissolving Ciprofloxacin   Mr. Chirag Joshi
• Transient transfection????   Roger Noble
• Assembling EST sequences   Thomas Newman
• Needed: mouse IgG2a expression vector   Bart Frank
• dialysis product needed   Jeffrey A. Cohlberg
• sequence dependent DNa structure   whst2 at cam.ac.uk
• An interesting puzzle - Pierce's "Gentle Ag/Ab elution buffer"? <-- SOLVED   Dima Klenchin
• expression array   Dr. N Kaushik
• enzymes immobilized on columns?   Marc Crepeau
• clone ring   Qunfeng Dong
• UNTRANSFORMED PLANTS RESISTANT TO HYGROMICIN   Jose Alfredo Iglesias Jimenez
• "Rinse the pellet"   Rocky Baker
• Chemiluminescent nucleic acid detection kit   Denis M. Larkin
• Very sensitive sulfhydryl assay?   cory
• FISH   Sarah Newlands
• Recombinant Vaccinia with T3 RNA polymerase   Smoothies
• Protease assay   Lars Komorowski
• "Rinse the pellet"   S. H.
• single nucleotide polymorphisms   Hiljar Sibul
• cardio supplements   cardio3 at juno.com
• Amersham Nylon Membranes   Michael Allen
• did phage-display cDNA library screening ever work?   Thomas Seib
• dialysis product needed   D. KIM
• dialysis product needed   D. KIM
• Dissolving Ciprofloxacin   Philip Hofstrand
• enzymes immobilized on columns?   Vladimir Svetlov
• "Rinse the pellet"   John Richard Seavitt
• dialysis product needed   Jeffrey A. Cohlberg
• dialysis product needed   Caroline Szymeczek-Seay
• MOPS   Caroline Szymeczek-Seay
• oxoid agar   Edith Duerbaum
• KCL staining of proteins in PAA gels   ldarj at MAIL.UTEXAS.EDU
• Very sensitive sulfhydryl assay?   Neil Pomroy
• bovine Cot-1 DNA   Wes Garrett
• Nucleovision gel documentation   Shiao Y. Wang
• Bandshift assay   Elsbeth Walker
• Bandshift assay   Jakobs
• Mutiple Shadow bands on seq gel   Thomas E Darga
• Cloning sticky PCR products (Breakthrough?)   Jim Graham
• secondary bands after in vitro transcription   schmitz
• Bandshift assay   Asteras Amaliadas
• help needed for getting pCMV5 sequence   Dr. Zhao Zhuoshen
• relationship between GST and c-fos   Kim sihyun
• enzymes immobilized on columns?   fwhyde at ibm.net
• How to eliminate phages   Heiko Koch
• polyacrylamide gel with formamide added???   Rachael Woram
• Restriction enzyme cutting frequency   Peter Lawson
• mouse spermatogenesis   sandro
• mouse spermatogenesis wrong address!!   Jim
• DNA microarray   Dr. N Kaushik
• enzymes immobilized on columns?   Susan Jane Hogarth
• DEGRADATION (RNA)   David O'Neill
• Quick Isolation Q   David O'Neill
• DNA Ligase in 2nd Strand Buffer. Why?   Warren Gallin
• DNA Ligase in 2nd Strand Buffer. Why?   Jeremy A. Guggenheim
• DNA Ligase in 2nd Strand Buffer. Why?   guggenheim at cf.ac.uk
• Starch detection by iodine   Lars Komorowski
• DNA microarray   R.Billeter
• microarrays   M.Wittwer
• cutting frequency of restriction enzymes   Peter Lawson
• Very sensitive sulfhydryl assay?   Shaun D. Black
• GET MONEY QUICK   socrates at super.net.uk
• DNA microarray   M.Wittwer
• DNA microarray   David de Graaf
• need protocol for EGFP extraction!   Richard Converse
• postdoc in Finland   Kristina Lindström
• DNA Ligase in 2nd Strand Buffer. Why?   Hiranya Roychowdhury
• Cloning sticky PCR products   Brian Cohen
• looking for Agrobacterium strain   techy
• Dissolving Ciprofloxacin   Kiley R. Prilliman
• Make Your Own Vacuum Pump!   z2154498 at student.unsw.edu.net
• DNA Ligase in 2nd Strand Buffer. Why?   Dr. Duncan Clark
• Amersham Nylon Membranes   Clemens Suter-Crazzolara
• Information on CMP-KDO synthetase assay method   Jose
• How to eliminate phages   Chris Boyd
• Que] Help!!! Putative S.D Region(RBS)   Hyunmin Kim
• enzymes immobilized on columns?   fwhyde at ibm.net
• DNA depurination   elebae at utu.fi
• mouse spermatogenesis   roberto
• rat cDNA libraires   Dr. T.F. King
• Blotting from Acrylamide Sequencing Gel   DrglennK
• Cloning sticky PCR products   Jim Graham
• methods@net.bio.net   Parent, Eric
• DNA Ligase in 2nd Strand Buffer. Why?   Patrick Hurban
• DNA depurination   Raymond Dalgleish
• primer programs   fender
• primer programs   Caroline Szymeczek-Seay
• RNA extraction in mouse vs human   Binnie Betten
• Cloning sticky PCR products   Vladimir Svetlov
• secondary bands after in vitro transcription   Heinz J. Schaefers
• methods@net.bio.net   Hiranya Roychowdhury
• screening clones by RT-PCR   Mike Smith
• Oligos Central - directory of oligonucleotide synthesizing companies   Ransom Hill Bioscience
• RNA precipitation by LiCl   Ralf Badur
• TGA Lignin quantification   Ralf Badur
• What is the best method to store 35S-labeled riboprobe?   Jun-Ichi Aikawa
• What is the best method to store 35S-labeled riboprobe?   Jun-Ichi Aikawa
• Oligos Central - directory of oligonucleotide synthesizing companies   Chris Odt
• screening clones by RT-PCR   Caroline Szymeczek-Seay
• cloning problem   Marek Nelke
• bacterial stock storage   Gail Sudlow
• Postdoctoral position in Arginase Biology   Ramaswamy K. Iyer
• Invitrogen's Dipsticks   Scott Craig
• ANSWERS -419   28552.John.Dill
• shelf-life of 1st strand cDNA?   sullivan at gwu.edu
• primer programs   Keith James
• Dot- blots on glass slide   John Landers
• differential display   helene dauchel
• FISH   Dr Margaret A. Leversha
• Finding splice sites with GCG?   Sami K J Kukkonen
• Pierce snakeskin dialysis tubing   Robin Lee Tremblay
• shelf-life of 1st strand cDNA?   brett
• KCL staining of proteins in PAA gels   Emile Schiltz
• Cloning sticky PCR products   Vladimir Svetlov
• Comments sought on tube luminometers   panini at etsu.edu
• biotin labld probe for chick OK?   Richard Vernell
• differential display   Jun-Zhi Wei
• differential display   Jun-Zhi Wei
• Fidelity and yield of PCR with ITP?   Jack_Leonard at MILLIPORE.COM
• ELISA against GST-fusion: got a protocol for me?   Jim Bloor
• Exon orientation   Simon Hoffenberg
• Pierce snakeskin dialysis tubing   Richard J. Dudley
• question about thermocyclers   Cayetana Schluter
• cloning high molecular weight DNA   jramon at servidor.unam.mx
• KCL staining of proteins in PAA gels   Axel Schumacher
• Want feedback about a database I made   Blair's news
• Upgrading Endnote? Buyer beware   Haun, Randy
• DNA precipitation: EtOH vs. Isoprop   brett
• DNA precipitation: EtOH vs. Isoprop   Frederik Boernke
• Costs of making it from Scratch?   The Alexanders
• 4" CLITS LICKED BY HOT TEENS AND CELEBS!   ADULTS
• Costs of making it from Scratch?   my2cents
• Costs of making it from Scratch?   Zophonias O. Jonsson
• Co-transfection with pCMV5 plasmids (help!!)   Xiaomin Fan
• Upgrading Endnote? Buyer beware   Roger Murphy
• Very sensitive sulfhydryl assay?   Roger Murphy
• gst   Kim sihyun
• gst   Kim sihyun
• Upgrading Endnote? Buyer beware   Ian Davis
• beta gal side effects   Dr. J. Miano
• Q: site-directed mutagenesis of large DNA (>50 kb)   Tom Litjens
• For test only (no content).   Wen-Feng Chen
• Question on Replica plating   CVRL
• shelf-life of 1st strand cDNA?   Zbigniew Rudzki
• Question on Replica plating   Andrew Warkman
• Immunprecip. with anti-HIS6 ab   Thomas Urbig
• Control module for UV detector   Jonathan Kurtis, M.D./Ph.D.
• Liver nuclear extracts?   kathy gately
• Tangential flow filterss   Ian McFarlane
• How to isolate RNA from gel???   Juergen Schmitz
• Costs of making it from Scratch?   Hiranya Roychowdhury
• Extremophile Workshop (scholarships available)   Mr. Ken Carter
• Cloning sticky PCR products (Breakthrough?)   David L. Haviland, Ph.D.
• Removal of Ethidium Bromide from Skin   skorycd at ncaur.usda.gov
• differential display   erika
• Co-transfection with pCMV plasmids (help!!)   Xiaomin Fan
• TA cloning vector   Stephen Billington
• Embedding and sectioning woody samples   yuanxiang Zhou
• RNA precipitation by LiCl   Aydemir Akin, DVM
• DNA precipitation: EtOH vs. Isoprop   Gail Sudlow
• Efficiency of IRES (Internal Ribosome Entry Site) is high?   Jun-Ichi Aikawa
• Q: How to label the N-terminus of a protein selectively?   Wen-Feng Chen
• How to isolate colonies of HeLa cells?   Zhonglin Chai
• Criticisms of Ames mutagencitiy tests   Dr. S. Shapiro
• Want feedback about a database I made   Yang Sheng
• Embedding and sectioning woody samples   Juergen Schmitz
• anti-His antibody   Flore Bellefontaine
• Genomic DNA extraction   Panapat Uawithya
• Conjugation of a synthetic peptide to monoclonal antibody   Stephen Carleton
• Question on Replica plating   David Winterbourne
• anti-His antibody   Lars Komorowski
• Large Scale Facility   Yair M. Heimer
• Question on Replica plating   Mike Gruidl
• G418 selection for CHO-K1   Ian McFarlane
• Humogenizing mammacarcinomas using the medimachine from DAKO   DI Thomas Mohr
• Humogenizing mammacarcinomas using the medimachine from DAKO   DI Thomas Mohr
• HELP: Coli is rearranging recombinant plasmid   P.J. v. Santbrink
• help   Yong Fang
• the list of growth factor receptor expression in common cells.   Dr. Zhao Zhuoshen
• transformation of meningococci   Dr. Kris Partridge
• TA cloning vector   Heinz J. Schaefers
• beta gal side effects   Marieke R. Koedood Zhao
• clone manager   J. Hendriks
• subtraction (of an acorn)   J. Hendriks
• Antibodies from gel slices AND pGEM-T mutations   Oliver S. DePeyer
• clone manager   Caroline Szymeczek-Seay
• Antibodies from gel slices AND pGEM-T mutations   Dom Spinella
• Antibodies from gel slices   David L. Haviland, Ph.D.
• quoting reference from WWW query   J Loh
• pGEX-2TK request   Frederik Boernke
• request for a new methods plese!!   Parit Plainkum
• xylooligosaccharide purification   ¿ÀÈ­±Õ
• lipoxygenase, glucanses help....   Luis Velasquez
• Q: How to label the N-terminus of a protein selectively?   Roger Murphy
• anti-His antibody   Tim Barnett
• HELP: METHOD FOR ATP PURIFICATION NEEDED   David Ackerley
• RNA transfections   Paul Gorry
• Lac repressor interference(Lac Switch)   Marcelo
• nuclear extracts with detergent???   Martin Offterdinger
• Streptococcal crude extracts   Ola Ween
• Membrane-phospholipid fusion/integration   jan.morren at bio.kuleuven.ac.be
• Exon orientation(ת¼Ä)   SYSOP.bbs at DNS.CST.SH.CN
• Finding splice sites with GCG?(ת¼Ä)   SYSOP.bbs at DNS.CST.SH.CN
• Finding splice sites with GCG?(ת¼Ä)   SYSOP.bbs at DNS.CST.SH.CN
• Finding splice sites with GCG?(ת¼Ä)   SYSOP.bbs at DNS.CST.SH.CN
• bacterial growth medium   Hans-Peter Schmitz
• nuclear extracts with detergent???   Jakobs
• Labeling PCR primers   John Fellers
• heat stable rec A   Matthias Engel
• Crude Oil (Petroleum) Available   Julian Goldberg
• One of life's mysteries?   Michael O'Donohue
• RNA transfections   brett
• HELP: Coli is rearranging recombinant plasmid   Dr. Duncan Clark
• clone manager   Dr. Duncan Clark
• Antibodies from gel slices AND pGEM-T mutations   Dr. Duncan Clark
• Exon orientation   Robert M. Mihalek
• rat cDNA libraries   Dr. T.F. King
• One of life's mysteries?   Dr. Duncan Clark
• heat stable rec A   Dr. Duncan Clark
• Q:A method for multiplying lambda phages in E.coli   Claus Franzke
• Internal standard for western blot?   Todd Serulneck
• bacterial growth medium   Chris Boyd
• Tabacco genomic libraries   Mary-Jo Farmer
• subtraction (of an acorn)   Dr. N Kaushik
• Avoid some cheap oligos   Zheng-yu Peng
• Coiled-coil domains   Simon Hoffenberg
• HELP: METHOD FOR ATP PURIFICATION NEEDED   Zophonias O. Jonsson
• Estimate size differences (37bps) between 2 PCR products?   kathy gately
• Comments sought on tube luminometers   Jose C. Juarez
• quoting reference from WWW query   Ian A. York
• Labeling PCR primers   Ravi K. Ramachandran
• quoting reference from WWW query   S. H.
• Avoid some cheap oligos   Ransom Hill Bioscience
• Labeling PCR primers   Hiranya Roychowdhury
• Co-transfection with pCMV5 plasmids (help!!)   Alexey Merz
• HELP: Coli is rearranging recombinant plasmid   Alexey Merz
• transformation of meningococci   Alexey Merz
• which cyotkines as adjuvants for immunization of mice?   Thomas Schnibbe
• anti-His antibody   Bob Steinberg
• HELP: Coli is rearranging recombinant plasmid   Alexey Merz
• the amount of 35S-UTP to synthisize riboprobe   Jun-Ichi Aikawa
• RNA transfections   Hung-yiu Ho
• Avoid some cheap oligos   Susan Jane Hogarth
• Q: Serine Proteinase   Hyunmin Kim
• Costs of making it from Scratch?   Anne Gillen
• ? Difficulty with Factor Xa cleavage ?   Jongrak Kittiworakarn
• Labeling PCR primers   ELADER at AMBION.COM
• Avoid some cheap oligos   ELADER at AMBION.COM
• Removal of Ethidium Bromide from Skin   Ed Frank
• Question on Replica plating   Wendy Ingram
• HELP: Coli is rearranging recombinant plasmid   clemen suter-crazzolara
• bulk segregant analysis   Bettina Lehnhardt
• HELP: Coli is rearranging recombinant plasmid   Dr. Duncan Clark
• bulk segregant analysis   Bettina Lehnhardt
• Exon orientation   Chris Boyd
• Q: Serine Proteinase   J.R. Pelmont
• HELP: alpha-2-macroglobulin fast-form/slow-form   DI Thomas Mohr
• Question on Replica plating - Turbo refs   Wendy Ingram
• post-doc position   E.A.L. Biessen
• heat stable rec A   Alberto I Roca
• Plasmid Stability Test   ruben.varela-calvino at kcl.ac.uk
• Pierce snakeskin dialysis tubing   Chris Boyd
• ligation of bluntet fragments   Trygve Meum Eliassen
• ligation of bluntet fragments   Trygve Meum Eliassen
• RCAT (Rolling Circle Amplification Technology) ?   Toshihiro Watanabe
• HELP: Coli is rearranging recombinant plasmid   Michael O'Donohue
• HELP: Coli is rearranging recombinant plasmid   Alexey Merz
• Postdoc: ANTIBODY ENGINEERING   Tarran Jones
• anti-trypsinogen antibody   yannick sorlet
• transformation of meningococci   Joel Gagliardi
• How to isolate RNA from gel???   January Weiner
• RNA transfections   brett
• about purification of large plasmids   methods at 163.NET
• species homologue or new isoform Definition request   bradley turner
• DNA Polymerase   Troy Jesse
• HELP: Coli is rearranging recombinant plasmid   Beilin Zhang
• RapidCycler + Cycle Sequencing????   Dr. Robert Rumpf
• Costs of making it from Scratch?   Gerd
• immunodetection.....?   Darren Mark Manley
• Warning: ATCC in a total mess   Nospam at noway.com
• Avoid some cheap oligos   Genemed Synthesis
• use of S1 nuclease to blunt-end DNA   Marc Crepeau
• Boiling ethidium bromide gel   Jennifer Skerratt
• Removal of Ethidium Bromide from Skin   Bryant Fujimoto
• the amount of 35S-UTP to synthisize riboprobe   Ed Frank
• Warning: ATCC in a total mess   Ed Frank
• help with DNAse footprinting...   Richard Converse
• basic question on: transcription factor-DNA seq. recognition   Guido Hooiveld
• RapidCycler + Cycle Sequencing????   Mathieu Ingouff
• HELP: Coli is rearranging recombinant plasmid   Koen De Smet
• Plasmid Stability Test   Stephan
• Warning: ATCC in a total mess   Dr. Duncan Clark
• mouse spermatogenesis   Roberto Gualtieri
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• RapidCycler + Cycle Sequencing????   Mathieu Ingouff
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   David
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   brett
• genomic DNA contamination in cDNA preparation   TIM
• Ribokinase   Lena Zaitseva
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   Ian A. York
• Costs of making it from Scratch?   Ralf Oltmanns
• Q: Chemiluminescence and enhancers - prediction from structure?   wgschech at med.uni-tuebingen.de
• Q: Chemiluminescence and enhancers - prediction from structure?   wgschech at med.uni-tuebingen.de
• Q: Chemiluminescence and enhancers - prediction from structure?   wgschech at med.uni-tuebingen.de
• Plant RNA   michael Tilley
• [Q]: Alkaline hydrolysis of RNA   Ralf Oltmanns
• Boiling ethidium bromide gel   Hiranya Roychowdhury
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   Hiranya Roychowdhury
• anti-CD4, 8 antibodies   Anne Gatensby
• Q: Chemiluminescence and enhancers - prediction from structure?   wgschech at med.uni-tuebingen.de
• DO you HAVE mRNA or cDNA of murine SKIN ?   marcvdc at lmb1.rug.ac.be
• RapidCycler + Cycle Sequencing????   Mathieu Ingouff
• HELP: METHOD FOR ATP PURIFICATION NEEDED   Vladimir Svetlov
• DNA Polymerase   Vladimir Svetlov
• Warning: ATCC in a total mess   Vladimir Svetlov
• Boiling ethidium bromide gel   Vladimir Svetlov
• DNA Polymerase   clemens suter-crazzolara
• Boiling ethidium bromide gel   un691cs at genius.embnet.dkfz-heidelberg.de
• reporter genes   Eli Hestermann
• ethidium teabags ?   sshaw at BUSTOFF.BWH.HARVARD.EDU
• Warning: ATCC in a total mess   MIKEHOLLOWAY
• basic question on: transcription factor-DNA seq. recognition   Vladimir Svetlov
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   Jeffrey A. Cohlberg
• Q: Serine Proteinase   Thomas J. Dougherty
• ligation of bluntet fragments   Arnoud van Vliet
• HELP   hqwb
• help with DNAse footprinting...   Jakobs
• Boiling ethidium bromide gel   Stephan
• Boiling ethidium bromide gel   Wolfgang Schechinger
• Making cDNA for RT-PCR of N-terminal fragment   Harry Witchel
• Warning: ATCC in a total mess   Vladimir Svetlov
• Antibodies from gel slices AND pGEM-T mutations   Dima Klenchin
• FISH fixation of soil samples,   Tim Beckenham
• [Q]: Alkaline hydrolysis of RNA   Ned Mantei
• Boiling ethidium bromide gel   Hiranya Roychowdhury
• clone manager   Per Mygind
• anti-His antibody   Dima Klenchin
• AD: Reliability Engineer   chaser at CHASE25.COM
• Human cell line   Jose E. Mejia
• Requesting help with my research.   Sven Nottebaum
• purchasing transilluminators-advice sought.   alex dobrovic
• purchasing transilluminators-advice sought.   Ned Mantei
• Taquenase   Clemens Kittinger
• Boiling ethidium bromide gel   Dr. Duncan Clark
• Impact Factors   Rob
• BY2 transformation   David Humair
• Coiled-Coil Domain(ת¼Ä)   SYSOP.bbs at DNS.CST.SH.CN
• Zeocin selection in tissue culture cells   Simon Dawson
• granulosa cell RNA   r.l.bates
• Nuclease protection   Jean-Pierre de Villartay
• granulosa cell RNA   r.l.bates
• Sucrose density gradients.. HELP!!   A. Hatton
• BL21 question   Chen Ho An
• Human cell line   Wolfgang Schechinger
• BL21 question   Michael Benedik
• clone manager   Caroline Szymeczek-Seay
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   Shaun D. Black
• Boiling ethidium bromide gel   Vladimir Svetlov
• Need mRNA or cDNA clones of IL-2 and IL-6.   Qiaoping Yuan
• 3AT-like chemical against URA3?   Jess H. Leber
• S.O.S. IN TRANSFORMATION OF Arabidopsis y Nicotiana   Nestor Fabian Galvis Serrano
• DNA pooling.   Yankit Tam
• DNA quantification.   Yankit Tam
• single copy plant gene   Cayetana Schluter
• DNA pooling.   Beth Wapelhorst
• How is plasmid transformation controlled?   Charles A Miller
• clone manager   Chris Kafer
• How is plasmid transformation controlled?   Koen De Smet
• Boiling ethidium bromide gel   un691cs at genius.embnet.dkfz-heidelberg.de
• Boiling ethidium bromide gel   Clemens Suter-Crazzolara
• (none)   methods at 163.NET
• transfecting eucaryotics - what celly cycle phase is the best?   Wolfgang Schechinger
• Unusual behaviour of protein on SDS-PAGE gels - any suggestions?   Ian McFarlane
• (none)   Clemens Suter-Crazzolara
• Valuable science resource   corniche information
• granulosa cell RNA   r.l.bates
• enzymatic cleavage of DNA to small pieces (20-40bp)?   Frank O. Fackelmayer
• Copy number info   Andrea Zuccolo
• How is plasmid transformation controlled?   Ian A. York
• enzymatic cleavage of DNA to small pieces (20-40bp)?   Dom Spinella
• Boiling ethidium bromide gel   Martin Gerken
• Reusing agarose gels   Douglas Prasher
• northern and Biomax MS   MKROIHER
• Need expertise on DNA recombination mechanisms   Patrice Cote
• enzymatic cleavage of DNA to small pieces (20-40bp)?   Stewart P Johnson
• tetR antibody: desperately seeking some!   Heidi Moss
• Making cDNA for RT-PCR of N-terminal fragment   Pamela Norton
• Kozak in protein expression   Per-Anders Olsson
• ingredient of hybridization buffer   X. Y. Zheng
• quantitative pcr   Bertram Feil
• Kozak in protein expression   Wolfgang Schechinger
• Need mRNA or cDNA clones of IL-2 and IL-6.   mark
• ingredient of hybridization buffer   David L. Haviland, Ph.D.
• Boiling ethidium bromide gel   Vladimir Svetlov
• quantitative pcr   Jun Zhang
• Boiling ethidium bromide gel   Vladimir Svetlov
• Help on DNA polymrases   Wenjin Yu
• ingredient of hybridization buffer   Vladimir Svetlov
• DNA quantification.   Harry Witchel
• Looking for anti-actin Ab   Todd Serulneck
• Help needed for 293 stabletransfection   Todd Serulneck
• No Affiliation (was Re: DNA quantification.)   Ransom Hill Bioscience
• 3AT-like chemical, but for URA3 (not FOA!)   Jess H. Leber
• How is plasmid transformation controlled?   Eugene
• Human cell line   Eugene
• how to kill BamH1   AKi Hoji
• Staining fungal nucleoli   Austen
• Hela.T4 transfection   Alex Chang
• Hydrolase assays   Lars Komorowski
• Boiling ethidium bromide gel   Stephan
• ethidium teabags ?   Clair Lee
• MENDEL DATABASE ANNOUNCEMENT   dee.rawsthorne
• enzymatic cleavage of DNA to small pieces (20-40bp)?   Dr. Duncan Clark
• how to kill BamH1   Dr. Duncan Clark
• Human cell line   Wolfgang Schechinger
• RNA + PCR   Gary Paterson
• cDNA synthesis in high G/C regions   Jim
• RNA + PCR   Dr. Duncan Clark
• yeast 2-hybrid system   Frances Mcmillan
• looking for anti-spla2 antibody !!!   lj.dewindt at fys.unimaas.nl
• Need mRNA or cDNA clones of IL-2 and IL-6.   Clemens Suter-Crazzolara
• Prepoured Sequencing gels   W. Tuttle
• ingredient of hybridization buffer   Dr. N Kaushik
• ethidium teabags ?   Wolfgang Schechinger
• NaCl and culture medium   ruben.varela-calvino at kcl.ac.uk
• Prepoured Sequencing gels   Roger C. Green
• Boiling ethidium bromide gel   Vladimir Svetlov
• how to kill BamH1   Vladimir Svetlov
• rapid hybridization   MKROIHER
• single copy plant gene   soren k rasmussen
• No Affiliation (was Re: DNA quantification.)   Pamela Norton
• meaning of "synthetic lethality"   mlamphier at hotmail.com
• PCR dead ?   Thomas Kreuzer
• Scientific Misconduct, Thomas Jefferson University, and Roger J. Pomerantz   Ransom Hill Bioscience
• Microfuging RNA   Rocky Baker
• NaCl and culture medium   Michelle Gleeson
• HOT New XXX Site!!!   Adult XXX Announce
• meaning of "synthetic lethality"   John S Jacobs Anderson
• isolation of nuclear RNA   Oliver Politz
• Wanted: 2nd hand inverted microscope   Samuel Gray
• DNA quantification.   werner at inw.agrl.ethz.ch
• Boiling ethidium bromide gel   Stephan
• Costs of making it from Scratch? (Kit inflation)   Jim Graham
• PCR dead ?   J.M. Ossewaarde
• No Affiliation (was Re: DNA quantification.)   s535290 at aix1.uottawa.ca
• cellular infection   Lestrate Pascal
• Boiling ethidium bromide gel   Chris Boyd
• IMPACT kit problems   Jacqui Whiteway
• no affiliation with anything at all   brett
• salmon sperm DNA for ppt'n   Robert M. Mihalek
• meaning of "synthetic lethality"   David M. Eisenmann
• Direct Genomic DNA sequencing   Your Name Here
• meaning of "synthetic lethality"   Michael Dorsett Onken
• DNA isolatin from Soybean   Chittibabu Guda
• salmon sperm DNA for ppt'n   Dom Spinella
• Flaws in this protocol?   U. Gausmann
• In situ hybridization methods   Ira M. Herman, Ph.D.
• Boiling ethidium bromide gel   Vladimir Svetlov
• Boiling ethidium bromide gel   Vladimir Svetlov
• Looking for anti-actin Ab   Christopher Jennings
• gelatin   Carhuaz Rina
• Looking for anti-actin Ab   Louis Hom
• Costs of making it from Scratch? (Kit inflation)   Marc Crepeau
• Reverse PCR   Hong Qin
• Digital camera   Shinhan
• gelatin   Wolfgang Schechinger
• how to kill BamH1   Mark Silby
• Digital camera   Wolfgang Schechinger
• Costs of making it from Scratch? (Kit inflation)   Wolfgang Schechinger
• Boiling ethidium bromide gel   Stephan Heeb
• Costs of making it from Scratch? (Kit inflation)   Warren Gallin
• northern and Biomax MS   Chris Odt
• Costs of making it from Scratch? (Kit inflation)   Ransom Hill Bioscience
• Costs of making it from Scratch? (Kit inflation)   David L. Haviland, Ph.D.
• Stable Transfectants   Schmitz
• Help on DNA purification for PCR   zhan Xu
• Trypsin Mapping   Darren Boehning
• Prepoured Sequencing gels   Arnoud van Vliet
• salmon sperm DNA for ppt'n   Arnoud van Vliet
• Costs of making it from Scratch? (Kit inflation)   Ransom Hill Bioscience
• Assay Idea-yes. it's called immuno-PCR   alex dobrovic
• salmon sperm DNA for ppt'n   ELADER at AMBION.COM
• granulosa cell RNA   ELADER at AMBION.COM
• Help with Mycobaterial DNA Prep   genesis

Last message date: Thu Apr 30 22:40:07 EST 1998
Archived on: Sun Mar 6 21:44:13 EST 2005

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