Protection of n-terminus during SDS-PAGE?

Richard Dudley RDUDLEY at PGH.AUHS.EDU
Mon Aug 3 09:21:32 EST 1998


I once read somewhere that if you want to sequence proteins after SDS-PAGE, you should use thioglycolic acid during electrophoresis to protect the N-terminus.  Has anyone ever tried this, or does anyone have a reference for this?

Alternatively, does anyone have soem good advice to offer on protecting the N-terminus?

Thanks!

rich
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         



More information about the Methods mailing list