Blotting onto PVDF

Karen spink pdxkgs at pdn1.gene.nott.ac.uk
Mon Aug 3 03:59:42 EST 1998


Has anyone had any problems Western blotting onto PVDF in CAPS buffer? I am
trying to get a sequence on my protein and it appears that the efficiency
of transfer is letting me down. Could it be the buffer, (10mM CAPS, 10%
methanol, pH 11) ? I have had no problems blotting onto nitrocellulose
using tris-glycine buffers.

Thanks for your time

Karen Spink



More information about the Methods mailing list