Blotting onto PVDF

Karen spink pdxkgs at
Mon Aug 3 03:59:42 EST 1998

Has anyone had any problems Western blotting onto PVDF in CAPS buffer? I am
trying to get a sequence on my protein and it appears that the efficiency
of transfer is letting me down. Could it be the buffer, (10mM CAPS, 10%
methanol, pH 11) ? I have had no problems blotting onto nitrocellulose
using tris-glycine buffers.

Thanks for your time

Karen Spink

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