plamid prep

Frederik Boernke boernke at nospam.ipk-gatersleben.de
Wed Aug 5 10:47:22 EST 1998


Dom Spinella wrote:
> 
> > I need to prepare 50mg plasmid DNA. Last year, I prepared 20mg of that which
> > took me 2 whole months. I really do not want to do it again by myself (it made
> > me sick). Is there anyone know any commercial company that could do this for me
> > (and better with good price)? Thanks. Wilson (wzhou0 at pop.uky.edu)
> 
> We've used a company called Lofstrand Laboratories for commercial
> plasmid preps (telephone: 800-541-0362 -- no affiliation, etc.) and have
> been very happy with the quality.  Now 50 mg is a fair amount, so I
> don't know how much it would cost (whatever do you need that much
> plasmid for anyway?). In any case, they're probably at least worth a
> call. Cheers.
> --Dom Spinella
> 
> Aside to Hiranya:  In my hands a good plasmid prep gives 1-5 mg/liter of
> saturated bugs for real high copy number plasmids like pUC.  So 50 mg
> would require at least 10 liters (and maybe a lot more depending on the
> plasmid) of culture.  It certainly wouldn't take us months to process it
> all, but I don't think I could pull it off in half a day!  And you have
> to admit that after hours of sniffing that much E. coli even us crusty
> old vets get a little nauseated. -- D.

Hi!

Have a look at BioTechniques 24: 438 - 444 (March1998). They have this
incredible medium which they claim to yield as much as 48 ug/ml of a
high
copy plasmid such as pBluescript. So a little more than a litre would
be sufficient in this case?!;-)

Ricky


******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de



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