Exonuclease VII vs Mung Bean Nuclease

Csaba Kiss csakis at ki.se
Wed Aug 5 05:14:46 EST 1998


Hi!

I would like to know what the difference is between these 2 enzymes. Both
digest single stranded DNA but not double stranded DNA.  The exonuclease VII
is heat inactivatable, MBN is not.
Which is reliable? Some nucleases can chew up the whole sample if the
conditions are not optimal.
Can anybody suggest me which I should use for chewing up 5' overhangs
(around 20 bp) or as a matter of fact any kind of overhang? The resulting
mixture will be used in PCR in subsequent steps!!!


Csaba Kiss
csakis at ki.se
MTC Karolinska Institute
Sweden





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