protein concentration

D. Pearton pearton at saul5.u.washington.edu
Thu Aug 6 18:27:42 EST 1998


Leon Schurgers (l.schurgers at bioch.unimaas.nl) wrote:
: Hello,

: I have a protein which only dissolves in 8 M urea or very high or low
: pH. Does anyone know how to measure the protein concentration in one of
: these solutions?

There is a kit from Genotech called the "Dotmetric" system.  It uses
1 microlitre of solution and is not sensitive to urea, SDS or pH.  We've
had some good success with it.

BTW, I'm not affiliated with the company.

Yours,
Yak
--
Dave Pearton
pearton at u.washington.edu
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Down he sank in a chair-ran his hands through his hair-	+
     And chanted in mimsiest tones			+
Words whose utter inanity proved his insanity,		+
     While he rattled a couple of bones.		+
							+
"The Hunting of the Snark" Lewis Carroll		+
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