How to get rid of DNA from sample of western blot?

Platini Kwok platinik at glink.net.hk
Thu Aug 6 12:49:54 EST 1998


Hi,

Recently, I was involved in doing western blot on retina of SD rat and I
found that there was a lot sticky, jelly like structure after boiling
with TBS with SDS, triton x-100 buffer although I centrifuged it can
sonicate the supernatant, it still here. How should I do so that I can
get rid of it? These sticky material gave vertically line rather than
horizontal band in SDS page or NC membrane.

Thank you!




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