protein expression

JJ Miranda atlantis at
Fri Aug 7 01:48:31 EST 1998

OK, I know very little about this...

If I wanted to use a certain enzyme, how easy would it be to simply 
express the enzyme in something like E. coli?  If it has been published 
as being cloned with a known sequence, what steps does one need to take 
ahead of time?  In general, what's the usual protocol?

Sincere regards,
JJ Miranda

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