Kanamycin in media

Cayetana Schluter cschlute at sfu.ca
Fri Aug 7 21:42:34 EST 1998


Hello all,

I'm trying to do plant (ginseng) transformations and I'm using kanamycin
to select for the transformants. Does anybody know how long kanamycin can
remain in solid MS media (50mg/L) at room temperature before it breaks
down? Ginseng is rather slow in that it takes at least 3 weeks before the
tissue starts to callus, so the fewer times I half to transfer my explants
the better. 

Cayetana



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