cell lysis buffer

Arne Mueller amuelle3 at gwdg.de
Fri Aug 7 15:56:53 EST 1998


Dear Sabine,
I use the following buffer:

50 mM Hepes-KOH, pH 7,4
2 mM EDTA
0,25 M Sucrose

collect the cells with a "rubber-scraper" and homogenize the cells with
10-20 strokes with a 22G-gauge (the number of strokes depends on the
cell type, control it via Trypan Blue staining).
the cells will be lyzed, but transmembrane- and membrane associated
proteins will stay where they are supposed to be.

Bye, Ina 
sabine Hanelt wrote:
> 
> Hi netters,
> I need a buffer to lyse adherent cells on a petri dish
> without triton or another detergent.
> Thanks in advance
> Sabine

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