Large Scale Ligation.

Tam Anh Le leta at
Sun Aug 9 11:51:27 EST 1998

Hello all
    I have a question that I can not find any reference to,I am hoping that
some one in this group can give me some insight. I need to do a ligation,
where I need mg of the ligated product. There is potential problems as I see
it in the amount of ligase needed for one things, is there any reference out
there regarding parameters of doing such large amount of ligation ?

-Thanks in advance
Tam Anh Le
Clinical Neuroscience
St Vincent Hospital Melbourne.

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