DNA Detection Limits

rhmorse rhmorse at genbiosys.com
Mon Aug 10 12:45:44 EST 1998


I need to document the minimum levels of detection for DNA (both ssDNA and
dsDNA) in various detection systems.  Could you forward references for the
following?  DNA in:
	 	agarose gels stained with ethidium bromide viewed with instant film
	 	agarose gels stained with ethidium bromide viewed with CCD camera
	 	Southern transfer probed with 32P-labeled probe on membrane
		ABI 373/377 with fluorescent labels produced by ABI
		Pharmacia ALF with Cy 5.0 fluorescent label produced by AmershamPharmacia
		polyacrylamide stained with silver
Ideally the units of measure would be moles.

Thank you very much.
	
	
	
	



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