Dyes in sucrose gradient centrifugation

Wolfgang Schechinger wolfgang.schechinger at med.uni-tuebingen.de
Tue Aug 11 10:04:48 EST 1998


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We want to isolate cell membranes and other fractions by sucrose
gradient ultracentrifugation. 
Does it make sense to add a lipophilic dye (as Sudan Red) to enhance the
visibility of the plasma membrane fraction?

Any protocols, ideas and comments?

Your input is welcome!

Wolfgang Schechinger
University of Tuebingen, Germany
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