Cells with pET16b are not growing in liquid Medium

Matthias Drosten Matthias.drosten at rz.ruhr-uni-bochum.de
Wed Aug 12 08:00:48 EST 1998


		
Hello,
I have got the following problem:

After transforming a ligation with pET16b and an insert into XL1 blue
I get about 60 colonies (7 colonies at the religation control) on TBY
+ Amp.   The grew very slowly.  Transferring them into liquid medium
they are not growing at all (at the moment nothing had happened for  2
Days.). Transferring them to an agarplate they are growing again.

Has anyone an idea to solve this Problem?? I want to isolate the
plasmide to verify the ligation.

Thanks for help

Heiko

Heiko.Koch at ruhr-uni-bochum.de




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